Scientists efficiently transitioned the cell line to be utterly animal-free

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July 26, 2022

Tasgola Bruner 202-483-7382

Washington – A brand new paper from the PETA Science Consortium Worldwide eV and the Luxembourg Institute of Science and Know-how reveals easy methods to enhance analysis relevance and reproducibility by changing media containing serum extracted from the blood of unborn calves with animal-free media.

An estimated 1.8 million unborn calves are killed worldwide to provide fetal bovine serum (FBS), an indefinite combination of molecules, together with hormones, proteins and progress elements obtained from the blood of fetal calves. after their moms have been slaughtered for meals. FBS, which is utilized in laboratories to assist develop cells in vitro, is a supply of variability, contributing to the reproducibility drawback in analysis. Scientific organizations, such because the Group for Financial Co-operation and Growth and the European Union Reference Laboratory for Alternate options to Animal Testing, have inspired the shift from utilizing FBS to utilizing chemically outlined, animal-free media .

The paper, revealed in Toxicology in Vitro, describes the transition of A549 cells, a human lung cell line generally utilized in analysis, to cell tradition media with out FBS or different elements of animal origin. The success of this mission units a precedent for a similar transition for different cell varieties and has far-reaching implications for the sphere of in vitro analysis.

“Our research demonstrates the feasibility of utilizing animal-free media and offers a mannequin that may be replicated for the transition of different cell varieties to serum-free media,” says Dr Amy Clippinger, President of the Science Consortium. . “The usage of animal-free media has quite a few advantages and we encourage researchers to speculate on this alternative to extend the standard and reproducibility of research.”

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